Name: PCR Cabinet
Brand: YEKGLOBAL
SKU: 32
Availability: InStock

Question 1

What is YEK-PCR100 PCR cabinet and why is it used?

Accepted Answer

YEK-PCR100 is a specialized sterile work cabinet designed for PCR (Polymerase Chain Reaction) sample preparation in molecular biology and genetics laboratories. The 1000x600x700 mm work area provides sufficient space for PCR devices, micropipettes, and reaction tubes. HEPA-filtered (99.99% @ 0.3 µm) laminar airflow (0.45 m/s) and UV-C sterilization (30W, 254 nm) prevent PCR contamination. Designed as pre-PCR work area; guarantees protection from previous amplicon contamination. Critical for diagnostic laboratories, forensic laboratories, research institutes, and medical faculties.

Question 2

What is PCR contamination and how is it prevented?

Accepted Answer

PCR contamination is the contamination of new reactions by amplicon (amplified DNA) molecules remaining from previous PCR reactions. Even a few DNA molecules can lead to false positive results. YEK-PCR100 provides multi-layer protection: (1) HEPA H13 filter traps 99.99% of airborne DNA particles. (2) Laminar airflow prevents particle accumulation and creates continuous clean air circulation. (3) UV-C light (254 nm wavelength) photochemically fragments DNA and RNA. (4) Slight negative pressure provides isolation from external air. (5) Stainless steel interior surface allows easy cleaning and decontamination.

Question 3

How does the UV-C sterilization system work and how effective is it?

Accepted Answer

YEK-PCR100 is equipped with 30W power, 254 nm wavelength UV-C lamp. 254 nm is the maximum absorption wavelength for DNA and RNA nucleic acids (thymine dimer formation). UV-C inactivates genetic material by breaking DNA chains. Automatic sterilization program can be programmed for 15-120 minutes before and after work. Effectiveness: 30 minutes UV-C exposure reduces surface DNA contamination by >99.9%. However, UV-C cannot penetrate shadowed areas and deep cracks, so combination with chemical cleaning (DNA decontamination solution) is recommended. Lamp life ~8000 hours, should be replaced annually.

Question 4

What operations should and should not be performed in PCR cabinet?

Accepted Answer

MUST DO in YEK-PCR100: master mix preparation, primer and probe addition, DNA/RNA template addition (low copy number), closing and labeling reaction tubes, preparing new chemical aliquots. MUST NOT DO: opening or analyzing PCR products (amplicon) (post-PCR operations should be done in separate room), electrophoresis gel loading, working with plasmid or high-copy DNA preparations, using inside PCR device (reaction preparation only). Workflow principles: unidirectional flow (pre-PCR → PCR → post-PCR) minimizes cross-contamination risk. Separate pipette sets and laboratory coats should be used.

Question 5

How is daily and periodic maintenance of PCR cabinet performed?

Accepted Answer

Daily maintenance (after each use): 15 minutes UV-C sterilization before opening cabinet, wiping work surface with 70% ethanol, then treatment with DNA decontamination solution (e.g., DNA Away, RNase Zap), checking airflow (with smoke test). Weekly maintenance: vacuuming HEPA filter pre-filter, visual inspection of UV lamp, cleaning glass panels. Monthly maintenance: measuring airflow velocity (with anemometer, target 0.45 m/s), detailed interior and exterior cleaning of cabinet. Annual maintenance: verifying HEPA filter efficiency with DOP test (must be >99.99%), replacing UV lamp (after 8000 hours), motor and fan check, calibration certification.

Question 6

What should be considered when purchasing PCR cabinet?

Accepted Answer

Criteria to consider when selecting PCR cabinet: (1) HEPA filter class: minimum H13 (99.95%), ideal H14 (99.995%). (2) UV-C power: at least 30W, 254 nm wavelength. (3) Airflow velocity: 0.40-0.50 m/s adjustable laminar flow. (4) Work area: at least 1000 mm width (for two-pipette work). (5) Noise level: <55 dB (for long work comfort). (6) Material: interior surface stainless steel or UV-resistant plastic. (7) Certification: ISO 14644 (Clean Room Standard), CE marking, NSF/ANSI 49 (Biosafety Cabinets classification, optional). (8) Warranty and service: minimum 2-year warranty, spare parts supply, local technical service support. (9) Price: $2000-5000 range (varies by features).

PCR Cabinet

Product Description

Sterile Environment for DNA/RNA Work

Contamination Prevention Technology

Powerful UV-C Sterilization

Negative Pressure System

Technical Specifications

Technical Specifications

Product Information

Frequently Asked Questions About PCR Cabinet